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CHARACTERIZATION OF HIGH AFFINITY CHOLINE UPTAKE BY TORPEDO CALIFORNICA T‐SACS
Author(s) -
Rothlein Joan E.,
Parsons Stanley M.
Publication year - 1979
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1111/j.1471-4159.1979.tb05263.x
Subject(s) - torpedo , depolarization , choline , chemistry , biophysics , electric organ , biochemistry , biology , receptor , acetylcholine receptor
— The high affinity choline uptake system present in T‐sacs prepared from Torpedo californica electric organ was shown to be insensitive to external Ca 2+ and to be absolutely dependent on external NaCl, with optimal uptake at approx 200 mM‐NaCl. Both Na + and Cl − separately stimulate uptake. Uptake also exhibited an optimum at approx 10mM‐external K + . Uptake was completely inhibited at 4°C. Approximately 50% of newly accumulated [ 3 H]choline was released by depolarization of T‐sacs regardless of the time or method of depolarization.