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FOUR PROTEIN POLYMORPHISMS IN PIGEON BRAIN EXTRACTS DETECTED BY TWO‐DIMENSIONAL GEL ELECTROPHORESIS
Author(s) -
Schenker T.
Publication year - 1979
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1111/j.1471-4159.1979.tb05248.x
Subject(s) - allele , phenotype , polymorphism (computer science) , microbiology and biotechnology , gel electrophoresis , genotype , electrophoresis , biology , chemistry , genetics , gene
— Proteins of the brain extracts of 85 individual pigeons ( Columba livia ) were mapped by two‐dimensional gel electrophoresis. The method is a modification of O'F arrell 'S technique and separates proteins first by charge and then by molecular weight. There were three proteins, A, B and D which had each a variant form. The positions of these six proteins on the gel corresponded to the following pH values and molecular weight values: protein A 1 , 6.4/43,000; A 2 , 6.6/43,000; B 1 , 5.7/41,000; B 2 , 5.8/40,000; D 1 , 6.2/22,000; D 2 , 6.2/21,000. The variants are genetically determined, since protein A, B and D each occurred in three phenotypes (A 1 , A 1 A 2 and A 2 ; B 1 , B 1 B 2 and B 2 ; D 1 , D 1 D 2 and D 2 ) corresponding to the three possible genotypes. From the observed frequencies of the phenotypes the following allele frequencies were calculated: allele A 1 , 72%; A 2 , 28%; B 1 , 15%; B 2 , 85%; D 1 , 74%; D 2 , 26%. A fourth protein named C occurred in four different forms (C 1 , 7.2/37,000; C 2 , 7.2/36,000; C 3 , 7.1/37,000; C 4 , 7.1/36,000) and six phenotypes (C 1 , C 1 C 2 , C 2 , C 1 C 3 , C 2 C 3 and C 4 C 3 ). This polymorphism is also interpreted as being genetically determined. The four alleles coding for the four protein C forms had the following frequencies: allele C 1 , 62%; C 2 , 27%; C 3 , 10.5%; C 4 , 0.5%.