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CERAMIDE‐GALACTOSYLTRANSFERASE AND CEREBROSIDE‐SULPHOTRANSFERASE LOCALISATION IN GOLGI MEMBRANES ISOLATED BY A CONTINUOUS SUCROSE GRADIENT OF MOUSE BRAIN MICROSOMES
Author(s) -
Siegrist H. P.,
Burkart T.,
Wiesmann U. N.,
Herschkowitz N. N.,
Spycher M. A.
Publication year - 1979
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1111/j.1471-4159.1979.tb05180.x
Subject(s) - cerebroside , galactosyltransferase , golgi apparatus , endoplasmic reticulum , microsome , membrane , biochemistry , myelin , ceramide , enzyme , chemistry , galactocerebroside , biology , ganglioside , oligodendrocyte , central nervous system , endocrinology , apoptosis
— A 17,000 g supernatant of mouse brain microsomes was subfractionated on a continuous sucrose gradient in order to localise ceramide galactosyltransferase (CGalt, EC 2.4.1.47) and cerebroside sulphotransferase (CST, EC 2.8.2.11), both enzymes involved in the synthesis of myelin lipids. The submicrosomal fractions were analysed for marker enzymes of myelin, plasma membranes, Golgi membranes, endoplasmic reticulum and lysosomes, and their protein distribution was studied. The results and EM studies give evidence that CGalT and CST are located in the Golgi membranes of the brain.