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AXONAL TRANSPORT OF LIPIDS IN THE RABBIT OPTIC SYSTEM
Author(s) -
Haley James E.,
Tirri Lawrence J.,
Ledeen Robert W.
Publication year - 1979
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1111/j.1471-4159.1979.tb04555.x
Subject(s) - superior colliculus , serine , chemistry , biochemistry , optic tract , axoplasmic transport , glycerol , optic nerve , lipid metabolism , biophysics , biology , microbiology and biotechnology , anatomy , retinal , enzyme
— Axonal transport of lipids was demonstrated in the rabbit optic system using [2‐ 3 H]glycerol and [3‐ 14 C]serine. Following intraocular injection of these precursors, radioactive lipids were detected in the optic tract, superior colliculus and lateral geniculate body over a 31 day period. The bulk of lipid appeared to migrate at a rate equivalent to that of rapidly transported protein which, when combined with a prolonged period of release into the axon, led to a peak of transported radioactivity at 6‐10 days for the 3 tissues. The suggestion of a second peak at 17 days indicated the possibility of a smaller slow component, although another interpretation is suggested. Analysis of individual transported lipids revealed [2‐ 3 H]glycerol to label phosphoglycerides preferentially and [3‐ 14 C]serine to be an effective precursor for sphingolipids and certain of the phosphoglycerides. [3‐ 14 C]Serine labeled axonally transported proteins to an even greater extent than lipids, revealing the same fast and slow components previously shown with other amino acids.