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CHARACTERIZATION OF THE BINDING OF [ 3 H]MUSCIMOL, A POTENT γ‐AMINOBUTYRIC ACID AGONIST, TO RAT BRAIN SYNAPTOSOMAL MEMBRANES USING A FILTRATION ASSAY
Author(s) -
Williams Michael,
Risley Edwin A.
Publication year - 1979
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1111/j.1471-4159.1979.tb04553.x
Subject(s) - muscimol , agonist , membrane , chemistry , synaptic membrane , binding site , gabaa receptor , ligand (biochemistry) , aminobutyric acid , biochemistry , receptor , biophysics , biology
— The binding of [ 3 H]muscimol, a potent GABA agonist, to crude synaptic membranes prepared from rat brain was studied using a filtration method to isolate membrane‐bound ligand. Specific binding was found to be saturable and occurred to two binding sites of K d 5 5 and 30 n m . Binding was Na + ‐independent and enhanced by both freezing and Triton treatment. Regional and subcellular distribution studies and pharmacological characterization of specific [ 3 H]muscimol binding are consistent with binding to the synaptic GABA receptor.