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STUDIES ON THE BIOCHEMICAL BASIS OF DISTAL AXONOPATHIES–I. INHIBITION OF GLYCOLYSIS BY NEUROTOXIC HEXACARBON COMPOUNDS
Author(s) -
Sabri M. I.,
Moore C. L.,
Spencer P. S.
Publication year - 1979
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1111/j.1471-4159.1979.tb04550.x
Subject(s) - glyceraldehyde 3 phosphate dehydrogenase , dehydrogenase , neurotoxin , dithiothreitol , glycolysis , biochemistry , lactate dehydrogenase , glyceraldehyde , enzyme , chemistry
— Neurotoxic hexacarbon compounds 2,5‐hexanedione (2,5‐HD) and methyl n‐butyl ketone (M n BK) inhibit crystalline and endogenous CNS and PNS glyceraldehyde‐3‐phosphate dehydrogenase (GAPDH). Preincubation of the enzyme with the toxin was necessary for inhibition. The enzyme activity of GAPDH was preserved by the addition of dithiothreitol in the presence of either neurotoxin. By contrast, lactate dehydrogenase (LDH) activity was not inhibited by these neurotoxic chemicals. Neurologically inactive compounds 1,6‐hexanediol and acetone failed to inhibit GAPDH. The present data indicate that 2,5‐HD and M “BK block energy metabolism by inhibiting glycolysis at the site of GAPDH. These observations may account for the known failure of GAPDH‐dependent axonal transport and the axonal degeneration which occurs in hexacarbon neuropathy.