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UNILATERAL BRAIN INJURY IN THE RABBIT; REVERSIBLE AND IRREVERSIBLE DAMAGE OF THE MEMBRANAL ATPases
Author(s) -
Rigoulet M.,
Guerin B.,
Cohadon F.,
Vandendreissche M.
Publication year - 1979
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1111/j.1471-4159.1979.tb00381.x
Subject(s) - ouabain , oligomycin , atpase , mitochondrion , edema , chemistry , medicine , cerebral edema , endocrinology , enzyme , biochemistry , sodium , organic chemistry
— Modifications of some membranal enzymatic activities in rabbit brain edema induced by cold injury were studied. The edema was characterized by the tissue H 2 O content and the K + /Na + ratio. Comparison of the respiratory rate of isolated mitochondria in the state 3 and 4 and the ADP/O ratio suggested an alteration in the ATP synthesis mechanism. The oligomycin sensitive ATPase activity was severely reduced in mitochondria isolated from edematous cells. The alteration of the ouabain sensitive Na + ‐K + ‐ATPase was first qualitative in the sense where the response of the ATPase to the K + /Na + ratio was modified. A loss of the total activity was then observed. Intravenous injection of CDP choline induced a regression of the edema, a restoration of the sensitivity of the mitochondrial ATPase towards oligomycin and a restoration of the sensitivity of the Na + ‐K + ‐ATPase to the K + /Na + ratio. These results suggest that the reversible damages of the cells induced by cold injury were due to a disorder at the protein‐lipid interaction level.