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POSTMORTEM AUTOLYTIC RESPONSE IN RAT BRAIN LYSOSOMES
Author(s) -
McKeown Stephanie R.
Publication year - 1979
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1111/j.1471-4159.1979.tb00362.x
Subject(s) - autolysis (biology) , postmortem changes , lysosome , time of death , biology , chemistry , microbiology and biotechnology , andrology , biochemistry , pathology , medicine , toxicology , enzyme
— Rat cerebral lysosomes were investigated during postmortem autolysis to determine the effect of the length and temperature of storage. A lysosome enriched fraction was isolated by differential ultracentrifugation and there was found to be a progressive increase in fragility as the length of storage increased. However, those lysosomes which survived the homogenisation procedure exhibited similar properties for at least 6 h after death if storage was at 21°C. Changes apparent after further storage for 18 h at 4°C were also detected in a shorter period if the temperature of storage was increased. The results suggest that, coupled with an all‐or‐none rupture of the lysosomes, there is a small increase in the permeability of the lysosomes shortly after death. In many respects the characteristics of the intact lysosomes up to 6 h after death resemble those obtained immediately.