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IN VIVO INHIBITION OF RAT BRAIN PROTEIN SYNTHESIS BY d‐AMPHETAMINE
Author(s) -
Roel L. E.,
Moskowitz M. A.,
Rubin D.,
Markovitz D.,
Lytle L. D.,
Munro H. N.,
Wurtman R. J.
Publication year - 1978
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1111/j.1471-4159.1978.tb12467.x
Subject(s) - in vivo , striatum , amphetamine , chemistry , polysome , protein biosynthesis , cerebral cortex , pharmacology , biochemistry , biology , endocrinology , medicine , dopamine , ribosome , rna , microbiology and biotechnology , gene
— Between 1 and 4 h after rats received a single injection of d‐amphetamine (15 mg/kg)(when brain polysomes are known to be disaggregated), the in vivo incorporation of [ 14 C]lysine into trichloroacetic acid‐precipitable brain protein was reduced by 28–48%. Incorporation of the 14 C label into the protein present in a 100,000 g supernatant extract of whole brain was similarly reduced (by 44%). Amphetamine administration suppressed protein synthesis in rat cerebral cortex, cerebellum, hypothalamus, striatum, and brainstem to an equivalent extent. The drug did not significantly affect lysine pool sizes measured in these brain regions; thus the reduced incorporation of labeled lysine was not the result of an isotope dilution effect. We therefore conclude that the brain polysome disaggregation resulting from amphetamine administration is associated with decreased in vivo synthesis of some brain proteins.