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TIME‐DEPENDENT PARTITIONING OF GLUCOSE CARBONS METABOLIZED BY THE SUPERIOR CERVICAL GANGLION FROM CALVES
Author(s) -
Quadflieg K. H.,
Brand K.
Publication year - 1978
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1111/j.1471-4159.1978.tb12450.x
Subject(s) - incubation , metabolism , chemistry , carbohydrate metabolism , biochemistry , citric acid cycle , pentose phosphate pathway , amino acid , chromatography , glycolysis
— Glucose metabolism in the superior cervical ganglion for calves has been studied by incubating slices with [1‐ 14 C]‐, [6‐ 14 C]‐ and [U‐ 14 C]‐labelled glucose at 37°C and pH 7.4. Glucose utilization and the metabolic partitioning of glucose carbon in products during different incubation periods ranging from 5 to 60 min were determined by isotopic methods. Separation and identification of labelled compounds have been achieved by anion and cation exchange chromatography as well as by TLC and enzymatic analyses. From the data obtained a carbon balance could be constructed showing lactate to be the major product of glucose metabolism followed by CO 2 and amino acids. Measuring the release of 14 CO 2 from differently 4 C‐labelled glucose, the existence of an active pentose phosphate pathway in the ganglion could be demonstrated although this pathway seems to contribute only to a small extent to glucose metabolism. The marked decrease of the C‐U: C‐6 and the C‐U:C‐1 ratios in 14 CO 2 observed in the course of incubation is discussed in terms of a time‐dependent change in the rate of synthesis of amino acids which are directly connected with intermediates of the citric acid cycle.