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A FLUOROMETRIC ASSAY FOR CHOLINE ACETYLTRANSFERASE and ITS USE IN THE PURIFICATION OF THE ENZYME FROM HUMAN PLACENTA
Author(s) -
Hersh Louis B.,
Coe Barbara,
Casey Lynette
Publication year - 1978
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1111/j.1471-4159.1978.tb12401.x
Subject(s) - choline acetyltransferase , enzyme , choline , biochemistry , chemistry , acetylcholine , placenta , enzyme assay , acetyltransferase , chromatography , human placenta , biology , acetylation , fetus , pregnancy , genetics , gene , endocrinology
— A fluorometric assay for choline acetyltransferase has been developed. This assay is based on coupling the choline acetyltransferase dependent formation of acetyl‐CoA from acetylcholine and coenzyme A, to the reactions catalyzed by the enzymes citrate synthase and malic dehydrogenase. Although this assay is not as sensitive as previously described radiometric assays, it can be conveniently used during enzyme purification. Employing this assay method, choline acetyltransferase has been purified from human placenta to a specific activity of 92.7 μmol acetylcholine formed/min/mg protein.