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REGULATION OF ACETYLCHOLINE SYNTHESIS: CONTROL OF CHOLINE TRANSPORT AND ACETYLATION IN SYNAPTOSOMES
Author(s) -
Jope R. S.,
Weiler M. H.,
Jenden D. J.
Publication year - 1978
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1111/j.1471-4159.1978.tb12386.x
Subject(s) - acetylcholine , choline , chemistry , pentobarbital , acetylation , synaptosome , biochemistry , biology , pharmacology , in vitro , gene
— The high affinity transport of choline (Ch) and the synthesis of acetylcholine (ACh) were measured in synaptosomes by measuring the utilization of [ 2 H 4 ]Ch. The synthesis of ACh was reduced under several conditions which reduce the availability of acetyl coenzyme A (AcCoA) including no glucose added, replacement of glucose with succinate or impairment of glucose utilization by bromopyr‐uvate, NaCN, or pentobarbital. These conditions did not reduce the amount of unacetylated [ 2 H 4 ]Ch in the synaptosomes indicating that the high affinity transport of Ch is not directly coupled to the synthesis of ACh.