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THE FLUX OF RADIOACTIVE LABEL THROUGH COMPONENTS OF THE SEROTONERGIC SYSTEM FOLLOWING THE INJECTION OF [ 3 H]TRYPTOPHAN: PRODUCT–PRECURSOR ANOMALIES PROVIDING EVIDENCE THAT SEROTONIN EXISTS IN MULTIPLE POOLS 1
Author(s) -
Lane J. D.,
Aprison M. H.
Publication year - 1978
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1111/j.1471-4159.1978.tb10770.x
Subject(s) - serotonin , serotonergic , tryptamine , tryptophan , 5 hydroxyindoleacetic acid , chemistry , tryptophan hydroxylase , 5 hydroxytryptophan , biochemistry , endocrinology , medicine , biology , amino acid , receptor
— Rats were given an intravenous injection of 100 μ Ci of l –[ 3 H]tryptophan and were killed between 5 and 180 min post–injection. Brains were dissected into seven areas, and specific activities were determined for major components (tryptophan, 5–hydroxytryptophan. serotonin and 5–hydroxy–indoleacetic acid) and detectable minor components (tryptamine, 5–methoxytryptamine. 5–hydroxyindo–lepyruvic acid, and 5–hydroxytryptophol) of serotonergic metabolism. The minor components represented small amounts of the total radioactivity with respect to the four major components. Anomalies in the product–precursor relationships for 5–hydroxytryptophan and serotonin, as well as for serotonin and 5–hydroxyindoleactic acid, suggest intraneuronal compartmentation of serotonin.