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DETERMINATION OF ACh CONCENTRATION IN TORPEDO SYNAPTOSOMES
Author(s) -
Morel N.,
Israel M.,
Manaranche R.
Publication year - 1978
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1111/j.1471-4159.1978.tb10492.x
Subject(s) - torpedo , chemistry , inulin , chromatography , volume (thermodynamics) , hemocytometer , analytical chemistry (journal) , biochemistry , physics , thermodynamics , receptor , acetylcholine receptor
— The concentrations of ACh and ATP of Torpedo electric organ synaptosomes were directly measured and found to be respectively 20.0 ± 6.4 mM and 3.1 ± 0.6 mM. The synaptosomal volume was estimated by a classical space marker technique using 14 C inulin and tritiated water. After counting the synaptosomes in an haemocytometer (and knowing their volume), a mean diameter of 3.5 p was calculated. The use of these classical techniques was rendered possible because of the homogeneity of the fraction and the large size of Torpedo synaptosomes.