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ELECTROPHORETIC ANALYSES OF PROTEINS TRANSPORTED TO THE RAT POSTERIOR PITUITARY
Author(s) -
Gainer Harold,
Brownstein Michael J.
Publication year - 1978
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1111/j.1471-4159.1978.tb10486.x
Subject(s) - chemistry , isoelectric focusing , fucose , biochemistry , posterior pituitary , methionine , electrophoresis , cysteine , pituitary gland , chromatography , glycoprotein , amino acid , hormone , enzyme
— [ 35 S]cysteine, [ 3 H]methionine, or [ 3 H]fucose were injected into the supraoptic nuclei (SON) of rats, and the labelled proteins that were transported to and accumulated in the posterior pituitary 24h post‐injection were analyzed electrophoretically. The transported, labelled proteins which were soluble in 0.1 m ‐HCl were primarily of low molecular weight (about 12,000 on SDS gels). However, the selectivity of labelling of these proteins by the three different labelled precursors could be revealed by isoelectric focusing. The 0.1 m ‐HCl insoluble labelled proteins, presumably reflecting membrane proteins transported from the SON to the pituitary, were more diverse and generally of higher molecular weight (> 43,000 on SDS gels).