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ACTIVATION OF GUANYLATE CYCLASE IN SYNAPTIC PLASMA MEMBRANES OF CEREBRAL CORTEX BY FREE FATTY ACIDS
Author(s) -
Asakawa T.,
Takenoshta M.,
Uchida S.,
Tanaica S.
Publication year - 1978
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1111/j.1471-4159.1978.tb07047.x
Subject(s) - linoleic acid , chemistry , arachidonic acid , biochemistry , oleic acid , gtp' , fatty acid , enzyme , medicine
— Guanylate cyclase (EC 4.6.1.2) of synaptic plasma membranes of rat cerebral cortex was stimulated about 6‐fold by several unsaturated fatty acids (arachidonic, linolenic, linoleic, oleic, palmitoleic and myristoleic acid). Ricinoleic acid (12‐hydroxyoleic acid) was much less effective. Saturated fatty acids (C 10 and C 14 ‐C 20 ) and the methylester of linoleic acid were ineffective. Stimulation by linoleic acid was influenced by the concentration of enzyme protein. At 480 μg/ml of protein 0.6 m m ‐linoleic acid produced maximal activation of 6‐fold_ Activity stimulated by linoleic acid examined with 1.0 m m ‐GTP was maximal at pH 7.8‐7.9 and with 2 m m ‐MnCl 2 , whereas basal activity showed broad optimal pH and Mn 2+ ‐concentration dependence. Activation of the enzyme by linoleic acid was only partially reversed by washing. Particulate guanylate cyclase of heart, small intestine, adrenal medulla, liver and lung was also activated by linoleic acid. The extents of activation (1.5‐14.7‐fold) by linoleic acid and the concentrations (0.2‐1.0 mat) required for maximal activation depended on the tissues.