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BLOCKADE OF FAST AXONAL TRANSPORT BY DIPHTHERITIC DEMYELINATION IN THE CHICKEN SCIATIC NERVE
Author(s) -
Kidman Antony D.,
Dolan Louise,
Sippe H. Jane
Publication year - 1978
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1111/j.1471-4159.1978.tb07034.x
Subject(s) - sciatic nerve , lesion , diphtheria toxin , spinal cord , anatomy , axoplasmic transport , axon , paresis , medicine , chemistry , neuroscience , pathology , biology , toxin , surgery , biochemistry
— Chicken sciatic nerves undergo demyelination following intraneural injection of diphtheria toxin due to a lesion at the site of injection. Paresis occurs after 1 week and lasts for approx 3 weeks; at the height of the lesion we injected [ 14 C]Ieucine into the ventral horn cells of the spinal cord and followed the axonal transport of fast flowing labelled proteins down the sciatic nerve fibres making measurements of flow rates at two different times. The results showed the fast flowing labelled proteins were blocked at the demyelination site. We measured total protein in the nerves and examined them histologically to confirm the lesion. Further studies are in progress on the post synaptic muscle cells and the impaired nerves.