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EFFECTS OF THE ANTICONVULSANT SODIUM VALPROATE ON γ‐AMINOBUTYRATE AND ALDEHYDE METABOLISM IN OX BRAIN
Author(s) -
Whittle S. R.,
Turner A. J.
Publication year - 1978
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1111/j.1471-4159.1978.tb06572.x
Subject(s) - aldehyde reductase , aldehyde dehydrogenase , chemistry , aldehyde , sepharose , anticonvulsant , reductase , biochemistry , enzyme , stereochemistry , biology , epilepsy , catalysis , neuroscience
— Succinic semialdehyde dehydrogenase and NADPH‐dependent aldehyde reductase have been purified from ox brain by affinity chromatography on 5′ AMP–Sepharose and 2′5′ ADP–Sepharose respectively. Aldehyde reductase has also been purified using chromatography on Procion Red HE3B–Sepharose. The effects of the anticonvulsant drug sodium valproate were examined on these enzymes, and also on GABA‐aminotransferase partially purified from ox brain. Aldehyde reductase was inhibited by valproate in an uncompetitive manner with respect to aldehyde substrates ( K i = 38–85 μM). The inhibitions of succinic semialdehyde dehydrogenase and GABA aminotransferase were approx 2 orders of magnitude weaker, suggesting that aldehyde reductase may be an important site of action of anticonvulsant drugs.