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DISTRIBUTION OF VASOACTIVE INTESTINAL POLYPEPTIDE (VIP) IN THE PORCINE CENTRAL NERVOUS SYSTEM
Author(s) -
Fahrenkrug J.,
Muckadell O. B. Schaffalitzky
Publication year - 1978
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1111/j.1471-4159.1978.tb06571.x
Subject(s) - vasoactive intestinal peptide , radioimmunoassay , hypothalamus , antiserum , endocrinology , central nervous system , medicine , vasoactive , cerebral cortex , distribution (mathematics) , hippocampus , chemistry , biology , peptide , neuropeptide , biochemistry , receptor , immunology , antibody , mathematical analysis , mathematics
— A sensitive and specific radioimmunoassay for vasoactive intestinal polypeptide (VIP) has been used to determine the regional distribution of the peptide in the porcine CNS. The hippocampus and cerebral cortex contained the highest concentration of VIP, while lower but significant amounts were present in the hypothalamus and neurohypophysis. The immunoreactive VIP in the brain appeared to be identical with the octacosapeptide originally isolated from porcine small intestine, since it was found to have identical chromatographic properties. to dilute in parallel with the standard curves and to react equally with six different VIP antisera independent of extraction procedure used.