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HISTAMINE‐SENSITIVE ADENYLATE CYCLASE IN HYPOTHALAMUS OF RAT BRAIN: H 1 AND H 2 RECEPTORS
Author(s) -
Portaleone P.,
Pagnini G.,
Crispino A.,
Genazzani E.
Publication year - 1978
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1111/j.1471-4159.1978.tb06562.x
Subject(s) - medicine , endocrinology , histamine , cyclase , adenylate kinase , stimulation , histamine h2 receptor , chemistry , cimetidine , receptor , hypothalamus , population , antagonist , biology , environmental health
— In in vitro experiments on rat hypothalamic homogenates the effects of biogenic amines such as histamine (HA), noradrenaline (NA), dopamine (DA), serotonin (5‐HT) and drugs such as isoprenaline (ISP), 2‐(2‐pyridyl)ethylamine (H 2 stimulant—H ls ), 4‐methyl‐histamine (H 2 stimulant H 2s ), mepyramine (H 1 antagonistp H la ), cimetidine (H 2 antagonist—H 2a ) were tested on adenylate cyclase activity. HA possessed a powerful stimulating effect on hypothalamic adenylate cyclase activity, higher than that shown by the other substances. The stimulating effect of HA was greatest in hypothalamic tissue from male rats, while tissue from females showed only a modest stimulation. H 2s , induced a greater stimulation of adenylate cyclase than H ls . On the other hand, the H 2a inhibited HA stimulation to a greater extent than the H la , H la and H 2a , when used together, completely inhibited the HA stimulation. HA may have a neurotrans‐mitter role in the hypothalamus, and in this area there appears to be a mixed population of H 1 and H 2 receptors, with a majority of H 2 receptors.