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HE TURNOVER RATE OF TUBULIN IN RAT BRAIN
Author(s) -
Forgue S. Thomas,
Dahl June L.
Publication year - 1978
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1111/j.1471-4159.1978.tb06254.x
Subject(s) - tubulin , leucine , tyrosine , half life , polyacrylamide gel electrophoresis , metabolism , chemistry , turnover , kinetics , biology , biochemistry , endocrinology , medicine , amino acid , microtubule , pharmacology , microbiology and biotechnology , pharmacokinetics , enzyme , physics , management , quantum mechanics , economics
The turnover rate of tubulin in rat brain was determined from the decay in specific radioactivity of the protein after pulse‐labeling. When precursors were administered by a parenteral route, the shortest half‐life, 9.8 days, was obtained with [ 14 C]NaHCO 3 ; the longer half‐lives obtained with [U‐ 14 C]glucose or [4,5‐ 3 H]leucine suggest significant reutilization of label. Furthermore, with leucine as precursor maximal specific radioactivity of tubulin was not obtained until eight days after administration of label. Labeling and decay kinetics obtained with [4,5‐ 3 H]leucine were markedly different when the isotope was administered directly into the lateral ventricle. The difference between the turnover rates of the ‐α and β subunits of tubulin purified by means of high resolution polyacrylamide gel electrophoresis was not statistically significant. A half‐life for tubulin of 6.2 days was measured by continuous intravenous infusion of [U‐ 14 C]tyrosine.