Premium
DNA‐DEPENDENT RNA POLYMERASES FROM RAT BRAIN, AND RNA SYNTHESIS IN GLIAL AND NEURONAL CELL‐ENRICHED FRACTIONS, AFTER PROTEIN RESTRICTION
Author(s) -
Kubaat.G. Birgitta,
Morean Marianne,
Decken Alexandra von der
Publication year - 1978
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1111/j.1471-4159.1978.tb06237.x
Subject(s) - rna , polymerase , sephadex , biology , microbiology and biotechnology , dna , biochemistry , enzyme , rna polymerase , gene
DNA‐dependent RNA polymerases were extracted from rat brain and separated by column chromatography on DEAE Sephadex into enzyme I and II. After 6 days of dietary protein restriction the α‐amanitin sensitive RNA polymerase II showed a decrease in activity per total brain wet weight, and per mg brain DNA. RNA polymerase I was unchanged under these conditions. The acid‐soluble pool of nucleotides was also unaffected. Separation of brain tissue into glial‐ and neuronal‐enriched cells revealed dietary effects on RNA synthesis in glial but not in neuronal elements. Brain wet weight and DNA content remained unaltered after 6 days of protein restriction: but body weight was reduced by 1.3 g day. Control rats increased in weight by 7.7 g/day.