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FAST AXOPLASMIC TRANSPORT OF A CALCIUM‐BINDING PROTEIN IN MAMMALIAN NERVE
Author(s) -
Iqbal Zafar,
Ochs Sidney
Publication year - 1978
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1111/j.1471-4159.1978.tb02656.x
Subject(s) - chemistry , axoplasmic transport , sephadex , calcium , sciatic nerve , biophysics , axon , anatomy , polyacrylamide gel electrophoresis , leucine , chromatography , biochemistry , biology , organic chemistry , enzyme , amino acid
— Calcium is transported at a fast rate of 410 mm/day in cat sciatic nerve on injection of 45 Ca 2+ into the L7 dorsal root ganglia. Nerve segments corresponding to the crest and the plateau regions of transported activity were analyzed by column chromatography on Sephadex G‐100 and Biogel A 5m columns and the fast transported 45 Ca 2+ found to be bound to a protein of 15,000 dalton. Using [ 3 H]leucine as a precursor, a labeled calcium binding protein (CaBP) was found located at the same position in elution volumes from the columns as was the protein‐bound 45 Ca 2 + . The level of [ 3 H]‐labeled CaBP in the crest and plateau regions were compared using column chromatography and polyacrylamide gel electrophoresis techniques and approx 3×4 times more [ 3 H]‐labeled activity was found in the crest as compared to the plateau. These findings indicate that Ca 2+ is fast transported in association with the CaBP. The relation of CaBP to the transport filament model of axoplasmic transport and its possible role in nerve are discussed.