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ON THE UPTAKE OF INOSITOL BY RAT BRAIN SYNAPTOSOMES
Author(s) -
Warfield Anna,
Hwang Shing Mei,
Segal Stanton
Publication year - 1978
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1111/j.1471-4159.1978.tb00133.x
Subject(s) - inositol , ouabain , synaptosome , cytochalasin b , biophysics , chemistry , biochemistry , substrate (aquarium) , membrane , biology , in vitro , sodium , receptor , ecology , organic chemistry
The uptake of inositol by rat brain synaptosomes occurs via an unsaturable process that even at substrate concentrations as low as 1 μM is unable to achieve a concentration gradient indicative of active transport. Dinitrophenol, ouabain and cytochalasin B did not affect uptake of the cyclitol. The data indicate that inositol uptake by rat synaptosomes occurs by diffusion or by a system with an affinity so low it is difficult to discern. The low capacity, saturable inositol uptake system observed in rabbit brain slices may reflect a species difference or uptake by elements of the slice other than neuronal membranes.