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RELATED CHANGES IN AMOUNTS OF ACh AND ATP IN RESTING AND ACTIVE TORPEDO NERVE ELECTROPLAQUE SYNAPSES
Author(s) -
Israel M.,
Lesbats B.,
Manaranche R.,
Marsal J.,
MastourFrachon P.,
Meunier F. M.
Publication year - 1977
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1111/j.1471-4159.1977.tb12319.x
Subject(s) - neurotransmission , acetylcholine , synaptic vesicle , postsynaptic potential , biophysics , depolarization , free nerve ending , adenosine triphosphate , chemistry , torpedo , vesicle , biology , biochemistry , endocrinology , membrane , acetylcholine receptor , receptor
— Closely related changes in the levels of acetylcholine (ACh) and adenosine triphosphate (ATP) in the electric organ of Torpedo exist during rest and synaptic activity. The present work clarifies these relations by showing:1 That both substances are involved in an oscillatory process induced by nerve stimulation. 2 Both substances are present in synaptic vesicles; the size of the bound pool of ACh is Ca 2 ‐dependent and is large when Ca 2+ is low. Free ACh and transmission are restored when Ca 2+ is present in the incubation medium. 3 The amount of ATP in the tissue is also Ca 2+ ‐dependent but is low when Ca 2+ is omitted. The addition of Ca 2+ to the physiological medium restores the amount of ATP in the tissue. 4 There is a postsynaptic release of ATP triggered by transmitter depolarization. This release was measured after single nerve impulses. 5 When added to the incubation medium, nucleotides strongly inhibit transmitter release. It is suggested that the postsynaptic release of ATP regulates transmitter release.