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IN VIVO RNA SYNTHESIS WITHIN THE RAT NODOSE GANGLIA
Author(s) -
Gunning P. W.,
Kaye P. L.,
Austin L.
Publication year - 1977
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1111/j.1471-4159.1977.tb12315.x
Subject(s) - rna , in vivo , ribosomal rna , nucleic acid , transfer rna , in vitro , biology , biochemistry , 18s ribosomal rna , microbiology and biotechnology , chemistry , genetics , gene
— The in vivo synthesis of RNA in the rat nodose ganglia has been studied following the intravenous introduction of ortho‐[ 32 P]phosphate. Analysis of the labelled RNA upon 2.2% polyacrylamide gels was performed. Rapidly‐labelled heterodisperse RNA. with a size range of 10S‐30S was detected after 3 h exposure to the isotope. Two peaks, of size 28S and 12S‐14S. were also observed. The former is thought to be processed 28S rRNA whereas the latter is consistent with its designation as ‘message‐like’ RNA (mlRNA). A rapidly turning‐over phosphorylated non‐nucleic acid contaminant prevented clear interpretation of the 4S region of the gel at short labelling times. However, this material was not present when the exposure time was increased to 24 h. At this time, only the stable RNA species 28S and 18S rRNA and 4S tRNA, were detected.