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ISOLATION AND IDENTIFICATION OF γ‐AMINOBUTYRYL‐CYSTATHIONINE FROM HUMAN BRAIN AND CSF
Author(s) -
Perry Thomas L.,
Hansen Shirley,
Schier Gary M.,
Halpern Berthold
Publication year - 1977
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1111/j.1471-4159.1977.tb10720.x
Subject(s) - peptide , cystathionine beta synthase , dipeptide , sephadex , human brain , autopsy , chemistry , biochemistry , brain chemistry , cerebrospinal fluid , amino acid , chromatography , biology , medicine , endocrinology , methionine , neuroscience , enzyme
— A new dipeptide, γ‐aminobutyryl‐cystathionine, has been identified in human brain and CSF. The compound was isolated from peptide concentrates which were prepared by removing free a‐amino acids from deproteinized brain extracts on a copper Sephadex column. The isolated peptide was shown to be GABA‐Cysta by standard chemical methods, and its identification was confirmed by mass spectrometry. GABA‐Cysta is present in both biopsy and autopsy specimens of adult human brain, its content in some brain areas being as high as 0.090 μrnol/g wet weight. Its concentration in CSF is much lower. What physiologic role this unusual peptide plays in brain remains to be determined.