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COMPARISON OF THE ISOELECTRIC AND MOLECULAR WEIGHT PROPERTIES OF TUBULIN SUBUNITS
Author(s) -
Feit H.,
Neudeck Ursula,
Baskin F.
Publication year - 1977
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1111/j.1471-4159.1977.tb10616.x
Subject(s) - isoelectric focusing , protein subunit , isoelectric point , polyacrylamide gel electrophoresis , gel electrophoresis , sodium dodecyl sulfate , chemistry , tubulin , electrophoresis , subspecies , biochemistry , chromatography , biology , genetics , enzyme , gene , microtubule , paleontology
— Brain tubulin subunits were separated by a combination of isoelectric focusing and electrophoresis in the presence of sodium dodecyl sulfate (SDS) using a two‐dimensional polyacrylamide slab gel technique. Isoelectric focusing separated tubulin subunits into two major groups of bands, such that the more acidic group corresponded to the α subunit and the less acidic group corresponded to the β subunit. In addition, isoelectric focusing resolved the β subunit into two subspecies which differed slightly in isoelectric properties but were the same apparent molecular weight. The a subunit was resolved into many subspecies that appear to differ from each other by both apparent molecular weight and isoelectric properties.