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CHARACTERIZATION OF A CHONDROITIN SULPHATE PROTEOGLYCAN FROM OVINE BRAIN
Author(s) -
White C. J. Branford,
Hudson Margaret
Publication year - 1977
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1111/j.1471-4159.1977.tb10429.x
Subject(s) - proteoglycan , chemistry , biochemistry , serine , cartilage , core protein , chondroitin , glycosaminoglycan , macromolecule , chromatography , biology , enzyme , extracellular matrix , anatomy
— A proteoglycan has been isolated from ovine brain tissue using a dissociative method of extraction. The preparation was then purified using ion‐exchange and gel chromatography, and an apparent molecular weight of 2 ± 10 6 was estimated. Chemical analysis together with β‐elimination studies strongly suggested that the macromolecule contains chondroitin sulphate chains covalently bound to a common protein core through a serine‐xylose linkage. Immunological studies showed that a common antigenic component exists between the respective protein moieties of the proteoglycan and a proteoglycan isolated from bovine nasal cartilage.