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MEASUREMENT OF BRAIN PROTEIN TURNOVER WITH [ 14 C]SODIUM BICARBONATE 1
Author(s) -
Chee Po Yok,
Dahl June L.
Publication year - 1977
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1111/j.1471-4159.1977.tb10425.x
Subject(s) - half life , tyrosine , sodium bicarbonate , bicarbonate , chemistry , microsome , amino acid , radiochemistry , sodium , chromatography , biochemistry , endocrinology , medicine , biology , enzyme , pharmacokinetics , organic chemistry
— Protein turnover in rat brain was measured over a period of 30 days by following the decay in specific radioactivity of acidic amino acids in proteins labelled by a single intraperitoneal injection of [ 14 C]NaHCO 3 . Two major populations of brain proteins can be identified from the resultant non‐linear decay curve—one with an average half‐life of 4 days and another with an average half‐life of 12 days. The half‐lives of total brain, mitochondrial, microsomal and soluble proteins determined over a period of 5 days were 3.4, 5.8, 2.8, and 2.6 days, respectively. Turnover of these same brain subcellular fractions was also measured by continuous infusion of [ 14 C]tyrosine. The estimated half‐lives were in close agreement with those obtained from the 5 day measurement of radioactive decay following a pulse label of [ 14 C]NaHCO 3 .