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RAPID CONCURRENT AUTOMATED FLUORIMETRIC ASSAY OF NORADRENALINE, DOPAMINE, 3, 4‐DIHYDROXYPHENYLACETIC ACID, HOMOVANILLIC ACID AND 3‐METHOXYTYRAMINE IN MILLIGRAM AMOUNTS OF NERVOUS TISSUE AFTER ISOLATION ON SEPHADEX G10
Author(s) -
WESTERINK B. H. C.,
KORF J.
Publication year - 1977
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1111/j.1471-4159.1977.tb07788.x
Subject(s) - homovanillic acid , dopamine , chromatography , chemistry , sephadex , 3,4 dihydroxyphenylacetic acid , biochemistry , enzyme , endocrinology , biology , serotonin , receptor
— A semi‐automated fluorimetric assay technique for the concurrent estimations of noradrenaline, dopamine, 3, 4‐dihydroxyphenylacetic acid, homovanillic acid and 3‐methoxytyramine is described. The method is based on a rapid manually performed isolation of the catecholamines and metabolites on small columns of Sephadex G10, followed by automated fluorimetric dection in continuous flow systems. Samples containing no more that 2‐5 ng (NA, DA, DOPAC or HVA) or 10 ng 3‐MT could be reproducibly measured. The small Sephadex G10 columns has proven to be an excellent alternative to the currently used columns of alumina or ion‐exchange resins.