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THE METABOLISM OF LABELLED CHOLINE IN NEURONAL AND GLIAL CELLS OF THE RABBIT IN VIVO 1
Author(s) -
Francescangeli E.,
Goracci G.,
Piccinin G. L.,
Mozzi R.,
Woelk H.,
Porcellati G.
Publication year - 1977
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1111/j.1471-4159.1977.tb07723.x
Subject(s) - choline , phosphatidylcholine , biochemistry , citicoline , in vivo , phosphorylcholine , plasmalogen , sphingomyelin , metabolism , biology , chemistry , phosphocholine , phospholipid , cholesterol , membrane , microbiology and biotechnology
— [Methyl‐ 3 H]choline has been injected intraventricularly into adult rabbits, and the rate of synthesis of phosphatidylcholine, choline plasmalogen and sphingomyelin (and their hydrosoluble precursors) in isolated neuronal and glial cells has been investigated. At all time intervals examined, the injected radioactivity was incorporated only into the base moiety of the choline lipids in both cell types. Maximum labelling of the two choline phosphoglycerides occurred in neurons 150 min after administration, whereas the highest specific radioactivity for glial phosphatidylcholine and choline plasmalogen was reached at 6 and 10 h, respectively. At any time interval examined, the neuronal and glial choline plasmalogen displayed a higher specific radioactivity than the corresponding diacyl‐derivative. The two phosphoglycerides incorporated the base in both cell populations at a faster rate than did whole brain tissue. Sphingomyelin was labelled in both cells at a low rate and acquired measurable radioactivity levels only after 2 h from isotope administration. Highest levels of radioactivity for phosphorylcholine and cytidine‐5′‐diphosphocholine were reached in both neurons and glia 1‐2 h after administration, but these levels per unit protein were higher in glial than in neuronal cells.