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DEVELOPMENT AND REGULATION OF LIPID SYNTHESIS FROM KETONE BODIES BY RAT BRAIN
Author(s) -
Patel M. S.,
Owen O. E.
Publication year - 1977
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1111/j.1471-4159.1977.tb07715.x
Subject(s) - ketone bodies , ketone , butyrate , chemistry , medicine , cerebral cortex , endocrinology , lipid metabolism , biochemistry , metabolism , biology , organic chemistry , fermentation
— Cerebral cortex slices from 1‐day old rats incorporated actively 3‐hydroxy[3‐ 14 C] butyrate into lipids. The rate of synthesis increased moderately during the first postnatal week and then decreased rapidly reaching negligible rates by the end of the fourth postnatal week. The incorporation of [3‐ 14 C]acetoacetate (5 mM) and 3‐hydroxy[3‐ 14 C]butyrate (5 mM) at non‐physiological concentrations into lipids by cerebral cortex from 1‐week old rats was markedly stimulated in the presence of added glucose; however, the addition of glucose had no effect on the oxidation of ketone bodies to 14 CO 2 At physiological concentrations of acetoacetate (0.5 mM) and 3‐hydroxybutyrate (1 mM), glucose stimulated both the oxidation of ketone bodies to 14 CO 2 and the incorporation of ketone body‐carbon into cerebral lipids. In contrast, the oxidation of [U‐ 14 C]glucose to CO 2 and its incorporation into lipids by brain slices were markedly reduced in the presence of ketone bodies at physiological as well as non‐physiological concentrations. However, the total rate of lipid synthesis, as determined by the incorporation of 3 H from 3 H 2 O, was maintained under these conditions. This indicated that the decreased contribution of the acetyl moiety from glucose was compensated to a similar extent by acetyl‐CoA formed from ketone bodies. The findings show that both acetoacetate and 3‐hydroxybutyrate are better precursors than glucose for in vitros lipid synthesis in developing rat brain.