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THE BINDING OF 5‐HYDROXYTRYPTAMINE TO ACIDIC LIPIDS IN ISOBUTANOL 1
Author(s) -
Johnson David A.,
Cho Tae Mook,
Loh Horace H.
Publication year - 1977
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1111/j.1471-4159.1977.tb06514.x
Subject(s) - chemistry , phosphatidic acid , isobutanol , dissociation constant , phosphatidylserine , tryptamine , lipophilicity , biochemistry , stereochemistry , chromatography , receptor , alcohol , membrane , phospholipid
— In order to examine the possibility that acidic lipids can account for the binding of 5‐hydroxy [ 3 H]tryptamine (5‐HT) to brain tissue, the binding to six acidic lipids was studied using an isobutanol‐water partition method. With the exception of the polyphosphoinositides, all the acidic lipids examined bind saturably and with high affinity. The apparent dissociation constants of 5‐HT to the acidic lipids were as follows: phosphatidylserine, 0.4 μM; phosphatidic acid, 0.6μM; diphosphoinositide, 0.8 μM; cerebroside sulfate, 1.4 μM; monophosphoinositide, 1.9 μM; and triphosphoinositide, 10 μM. The high affinity of these lipids to 5‐HT raises the possibility of some role for them in serotonergic activity.