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SUBCELLULAR FRACTIONATION STUDIES RELATED TO THE PROCESSING OF NEUROSECRETORY PROTEINS IN APLYSIA NEURONS
Author(s) -
Loh Y. Peng,
Sarne Y.,
Daniels M. P.,
Gainer H.
Publication year - 1977
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1111/j.1471-4159.1977.tb03935.x
Subject(s) - aplysia , endoplasmic reticulum , cell fractionation , biology , granule (geology) , microbiology and biotechnology , biochemistry , neuron , polyacrylamide gel electrophoresis , neuroscience , enzyme , paleontology
— Neurosecretory cells (bag cells and R 3–14 neurons) in the abdominal ganglion of Aplysia californica were ‘pulse‐chased’ in [ 3 H]leucine and comparisons of the labeled protein profiles from the total cell homogenate versus a crude ‘neurosecretory granule’ fraction on acid‐urea polyacrylamide gels were made, The data provides indirect support for the hypothesis that some of the post‐translational processing of the neurosecretory proteins occurs intragranularly (L oh et al , 1975). In the case of the Bag cells the initial processing of the 29,000 daltons precursor appears to occur extragranularly, possibly in the rough endoplasmic reticulum cisternae.