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ELEVATED INTRACELLULAR GLYCINE ASSOCIATED WITH HYPOXANTHINE‐GUANINE PHOSPHORIBOSYLTRANSFERASE DEFICIENCY IN GLIOMA CELLS 1
Author(s) -
Skaper S. D.,
Seegmiller J. E.
Publication year - 1977
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1111/j.1471-4159.1977.tb03927.x
Subject(s) - hypoxanthine guanine phosphoribosyltransferase , phosphoribosyltransferase , hypoxanthine , lesch–nyhan syndrome , glycine , hypoxanthine phosphoribosyltransferase , intracellular , biochemistry , biology , microbiology and biotechnology , enzyme , chemistry , amino acid , gene , mutant
— The intracellular concentrations of a number of amino acids were measured in a normal clone of rat glioma cells, and in several independently derived clones selected for gross deficiency of the enzyme hypoxanthine‐guanine phosphoribosyltransferase (HGPRT). A significant, approx 2‐fold increase in the concentration of free glycine was observed in both mutagenized and non‐mutagenized HGPRT deficient clones. The increase in glycine was independent of the phase of cell growth. A similar increase did not occur in HGPRT deficient lymphoblasts.