Premium
INFLUENCE OF LIPIDS ON THE ACTIVITY OF CEREBROSIDE‐SULPHOTRANSFERASE IN MOUSE BRAIN: A COMPARATIVE STUDY OF JIMPY AND NORMAL MOUSE BRAINS 1
Author(s) -
Siegrist H. P.,
Burkart T.,
Steck A. J.,
Wiesmann U.,
Herschkowitz N. N.
Publication year - 1976
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1111/j.1471-4159.1976.tb12288.x
Subject(s) - cerebroside , microsome , chemistry , acetone , lecithin , substrate (aquarium) , stimulation , biochemistry , glucocerebroside , enzyme , chromatography , biology , endocrinology , glucocerebrosidase , ecology
— The effect of lipids other than the substrate cerebroside on the activity of cerebroside‐sulphotransferase (CST) in Jimpy and normal mouse brain was investigated. The enzyme activity of an acetone‐treated microsomal preparation can be stimulated in the presence of the extracted lipids either with or without addition of exogenous cerebroside as a substrate. The CST activity in the Jimpy mutant compared to that in normal animals differs from 18% in homogenate to approx 80% in solubilized or acetone‐extracted microsomes. An addition of total lipid from normal mouse brain to microsomal preparations from which lipid has been removed by acetone results in a stimulation of Jimpy CST activity up to a value of 80% of normal mouse brain microsomes. Both Jimpy and normal mouse brain CST can be also stimulated by the addition of single lipid components such as cholesterol and lecithin by 50% in normal and 100% in Jimpy brain microsomes. These findings lead to the hypothesis that there is a lipid requirement for CST activity other than the substrate cerebroside.