Premium
EFFECTS OF AMPHETAMINE ADMINISTRATION IN VIVO ON IN VITRO PROTEIN SYNTHESIZING SYSTEM FROM RAT BRAIN 1
Author(s) -
Widelitz Martin M.,
Coryell Marlene R.,
Widelitz Howard,
Avadhani Narayan G.
Publication year - 1976
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1111/j.1471-4159.1976.tb12270.x
Subject(s) - amphetamine , dopamine , in vivo , in vitro , chemistry , phenylalanine , pharmacology , serotonin , ribosome , protein biosynthesis , biochemistry , endocrinology , biology , amino acid , rna , receptor , microbiology and biotechnology , gene
— A highly active in vitro protein synthesizing system (S‐28) has been prepared from rat brain. Poly (U)‐dependent [ 3 H] phenylalanine incorporation by brain S‐28 system is significantly inhibited by D‐amphetamine. The extent of inhibition by amphetamine is significantly higher than by other biogenic amines such as dopamine and serotonin. At the 100°g level of amphetamine, the inhibition is about 70°. Experiments with ribosomes and soluble enzymes from control and amphetamine‐treated systems indicate that the observed inhibition may be due to the effect of the drug on the ribosomes. Kinetic analysis of the reaction mixture in the presence as well as absence of D‐amphetamine indicate that this sympathomimetic drug inhibits polysome formation in vitro.