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RAT BRAIN FOLATE IDENTIFICATION
Author(s) -
Brody T.,
Shin Y. S.,
Stokstad E. L. R.
Publication year - 1976
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1111/j.1471-4159.1976.tb12262.x
Subject(s) - folic acid , chemistry , biochemistry , medicine
— Endogenous rat brain folates were identified using column chromatography for folate separation, authentic folate standards, and microbiological assay. The total folate content of rat brain was 360 ng per gram brain (wet wt) and consisted of tetrahydropteroylpentaglutamate (H 4 PteGlu 5 , 29%), H 4 PteGlu 6 (14%), H 4 PteGlu 7 (7%), 5‐CH 3 ‐H 4 PteGlu 4 (7%), 5‐CH 3 ‐H 4 PteGlu 5 (13%), and small amounts of formyl‐, methyl‐, and H 4 PteGlu 1‐6 (~25%) and non‐methylated folates having a glutamate chain over seven glutamates long (4%). Overall folate oxidation state and one carbon forms were H 4 PteGlu n (58%), 5‐CH 3 ‐H 4 PteGlu n (18%), 5‐ and 10‐CHO‐H 4 PteGlu n (16%), and H 2 PteGlu n (8%). No PteGlu n was detected.