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A RAPID ASSAY FOR 2′,3′‐CYCLIC NUCLEOTIDE 3′‐PHOSPHORYDROLASE 1
Author(s) -
Sims N. R.,
Carnegil P. R.
Publication year - 1976
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1111/j.1471-4159.1976.tb10406.x
Subject(s) - coprecipitation , nucleotide , chemistry , adenosine , salt (chemistry) , adenosine monophosphate , nuclear chemistry , biochemistry , inorganic chemistry , organic chemistry , gene
— Separation of 2′‐adenosine monophosphate from 2′,3′‐cyclic adenosine monophosphate by coprecipitation with a number of salt mixtures was examined and found to be most successful with Na 2 CO 3 /CdCl 2 and Na 2 CO 3 /ZnSO 4 . A simple and rapid assay for 2′,3′‐cyclic nucleotide 3′‐phosphohydrolase using coprecipitation with Na 2 CO 3 /CdCl 2 is described.