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CHOLINE UPTAKE BY CHOLINERGIC NEURON CELL SOMAS
Author(s) -
Suszkiw J. B.,
Beach R. L.,
Pilar G. R.
Publication year - 1976
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1111/j.1471-4159.1976.tb06995.x
Subject(s) - choline , denervation , ouabain , ganglion , acetylcholine , cholinergic , superior cervical ganglion , chemistry , endocrinology , neuron , medicine , biophysics , biology , neuroscience , sodium , organic chemistry
The cellular compartments of ciliary ganglia take up choline by a single, saturable process with K m =7.1 × 10 −5 M and V max = 4.66 pmol/min per ganglion: Denervation of the ganglia and the resultant degeneration of nerve terminals caused no significant decrease of the rate of accumulation of choline by the ganglia. This indicates that the measured uptake is by the postganglionic ncurons and nonneural elements (NNE: glial and connective tissue cells) in the ganglia. This uptakc is not dependent on metabolic energy and is not affectcd by lowcring Na + or raising K + concentrations in the incubating mcdia but is depressed in the presence of ouabain and hemicholinium‐3. The presence or Na + ‐dependent. rapidly saturable uptake in the preganglionic nerve terminals which is not detectablc kinetically is, however, inferred from a decrease in ACh synthesis in dcncrvatcd prcparations and a similar decrcasc in intact ganglia incubated in low Na+ solution.