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UPTAKE OF TAURINE INTO SUBCELLULAR FRACTIONS OF C‐6 GLIOMA CELLS 1
Author(s) -
Sieghart W.,
Karobath M.
Publication year - 1976
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1111/j.1471-4159.1976.tb06481.x
Subject(s) - taurine , differential centrifugation , glioma , centrifugation , biochemistry , subcellular localization , cerebral cortex , cell fractionation , chemistry , enzyme , synaptosome , biology , chromatography , in vitro , cytoplasm , amino acid , endocrinology , cancer research
— Subcellular fractions from cultured C‐6 glioma cells prepared by methods similar to those for crude synaptosomal fractions of rat cerebral cortex accumulated [ 35 S]taurine as did intact glioma cells. Thus, the accumulation of taurine was dependent on temperature and sodium concentration and sensitive to osmotic shock. The kinetic properties of this uptake are characterized by an apparent K m , of about 25 μm, The properties of taurine uptake into subcellular fractions from C‐6 glioma cells were compared with those of crude synaptosomal fractions and differences could be observed in temperature sensitivity and with metabolic inhibitors, which were less potent in the glioma preparation. Equilibrium density gradient centrifugation of subcellular fractions from glioma cells revealed that particles containing [ 35 S]taurine sediment to a lower buoyant density than mitochondria. But on co‐sedimentation of subcellular fractions from glioma cells with synaptosomal fractions derived from cerebral cortex, differences in the buoyant density between these two preparations could be found. The findings support the possibility of a contamination of synaptosomal fractions with subcellular fractions derived from glial origin.