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ANALYSIS OF RNA SYNTHESIZED BY AN ISOLATED RAT BRAIN SYNAPTOSOMAL FRACTION
Author(s) -
England J. M.,
Attardi G.
Publication year - 1976
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1111/j.1471-4159.1976.tb05152.x
Subject(s) - rna , biochemistry , uridine , mitochondrion , fraction (chemistry) , biology , synaptosome , free nerve ending , microbiology and biotechnology , chemistry , in vitro , anatomy , chromatography , gene
— A synaptosomal fraction derived from rat brain, when incubated in an appropriate medium. incorporates [5‐ 3 H]uridine into RNA. On the basis of sedimentation analysis, RNA‐DNA hybridization and metabolic inhibitor studies, mitochondrial RNA species appear to be the major, if not sole, RNA products synthesized by the isolated synaptosomal fraction. Electronmicroscope autoradiographic analysis showed that about 50% of the incorporation of [5‐ 3 H]uridine into RNA by this fraction occurs in the presynaptic endings. The capacity of mitochondrial RNA synthesis in isolated nerve endings declines as the age of the animal increases from 10 to 30 days, and by 60 days of age discrete mitochondrial RNA species are barely detectable.