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THE BINDING OF ACETYLCHOLINE TO SYNAPTIC VESICLES FROM TORPEDO CALIFORNICA ELECTROPLAX
Author(s) -
Mattson M. E.,
O'Brien R. D.
Publication year - 1976
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1111/j.1471-4159.1976.tb05148.x
Subject(s) - torpedo , acetylcholine , vesicle , acetylcholine receptor , chemistry , muscarinic acetylcholine receptor , acetylcholinesterase , nicotinic agonist , synaptic vesicle , cholinergic , biochemistry , muscarinic acetylcholine receptor m5 , potency , biophysics , receptor , muscarinic acetylcholine receptor m3 , in vitro , biology , pharmacology , membrane , endocrinology , enzyme
— The in vitro uptake of exogenous acetylcholine by isolated presynaptic vesicles has been demonstrated in a new system. A preparation of vesicles from Torpedo californica electroplax was developed in which acetylcholinesterase and acetylcholine receptor activity were blocked. The vesicles bound acetylcholine with K d 1.58 μM, the maximum amount bound being 26 pmol per g of original tissue, or 52 molecules per vesicle. Nicotinic drugs blocked binding, but muscarinic and noncholinergic drugs did not. The relative potency of nicotinic drugs differed greatly from their potency on Torpedo receptor. Sephadex chromatography showed that 26% of the binding was irreversible. The relationship of the binding to acetylcholine uptake and storage was discussed.