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PHOSPHOLIPID METABOLISM IN SUBACUTE SCLEROSING PANENCEPHALITIS : ACTIVITY OF BRAIN PHOSPHOLIPASE A 2 TOWARDS SPECIFICALLY LABELLED 1,2‐DIACYL‐, 1‐ALK‐1′‐ENYL‐2‐ACYL‐ AND 1‐ALKYL‐2‐ACYL‐ sn ‐GLYCERO‐3‐PHOSPHORYLCHOLINE
Author(s) -
Woelk H.,
JakumeitMorgott U.,
Schenck K.
Publication year - 1976
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1111/j.1471-4159.1976.tb04476.x
Subject(s) - phosphorylcholine , phospholipid , phospholipase , subacute sclerosing panencephalitis , biochemistry , phospholipase a2 , chemistry , acyltransferase , plasmalogen , metabolism , moiety , phospholipase a1 , enzyme , hydrolysis , acylation , choline , phospholipase a , stereochemistry , biology , measles , membrane , immunology , measles virus , vaccination , catalysis
—1,2‐Diacyl‐, 1‐alk‐1′‐eny1‐2‐acyl‐ and 1‐alky1‐2‐acyl‐ sn ‐glycero‐3‐phosphorylcholine specifically labelled with different fatty acids at the 2 position, were prepared enzymically using the acyltransferase system of rabbit sarcoplasmic reticulum. The substrates were submitted to hydrolysis by phospholipase A 2 (phospholipid acyl‐hydrolase, EC 3.1.1.4) obtained from normal and brain tissue affected with subacute sclerosing panencephalitis. In the diseased tissue an increase of phospholipase A 2 activity ranging from 46 to 54% could be observed in comparison to the control brain for all substrates investigated. Among the investigated substrates phospholipase A 2 had the highest affinity for the 1,2‐diacylcompound, whereas alkenylacyl‐ and alkylacyl‐ sn ‐glycero‐3‐phosphorylcholine were cleaved at almost similar rates. The hydrolysis rate of choline‐plasmalogen and the corresponding diacyl compound by the enzyme was greatly influenced by the fatty acid moiety located at the 2 position of the substrates.