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THE NON‐SYNCHRONOUS SYNTHESIS OF MYELIN COMPONENTS DURING EARLY STAGES OF MYELINATION IN THE RAT OPTIC NERVE 1
Author(s) -
Detering Nancy K.,
Wells M. A.
Publication year - 1976
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1111/j.1471-4159.1976.tb04473.x
Subject(s) - myelin , polyacrylamide gel electrophoresis , endocrinology , chemistry , optic nerve , medicine , myelin sheath , electrophoresis , sodium , gel electrophoresis , proteolipid protein 1 , myelin basic protein , biochemistry , biology , central nervous system , anatomy , enzyme , organic chemistry
—Analysis of lipids and proteins in whole optic nerves during initial stages of myelination indicate a sequential deposition of myelin components. During this period sulfatide levels increase from 4 nmol/pair of nerves at 9 days to 29 nmol/pair of nerves at 21 days. Cerebrosides are not detected until the 12th day and attain a level of 46 nmol/pair of nerves in the 21‐day old rat. Plasma‐logen values increase from 2.1 to 31 nmol/pair of nerves and cholesterol values from 12 to 158 nmol/pair of nerves between 9 and 21 days. High molecular weight proteins are present in samples from 9‐day old rats analyzed by discontinuous sodium dodecyl sulphate‐polyacrylamide gel electrophoresis. Under identical conditions myelin basic proteins are first observed in the 10 day sample, and the proteolipid protein is first detected in the 12 day sample. The 2′, 3′‐cyclic nucleotide 3′‐phosphohydrolase activity increases from 1.13 to 5.50 μmol/min per mg protein between 9 and 21 days.