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A KINETIC ANALYSIS OF SODIUM DEPENDENT GLUTAMIC ACID TRANSPORT IN PERIPHERAL NERVE
Author(s) -
Wheeler D. D.
Publication year - 1976
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1111/j.1471-4159.1976.tb04471.x
Subject(s) - sodium , glutamic acid , chemistry , kinetics , kinetic energy , sodium glutamate , glutamate receptor , biophysics , biochemistry , amino acid , organic chemistry , biology , receptor , physics , quantum mechanics , raw material
—The kinetics of sodium dependent glutamic acid transport have been studied in desheathed frog sciatic nerve. Initial velocities have been measured as a function of both glulamic acid and sodium concentration. Lineweaver–Burk plots are constructed from these data, and the kinetic constants describing uptake are estimated. V max is unaffected by sodium concentration, which implies that translocation is not directly affected by sodium. K 1 is sodium dependent, which implies that sodium affects the affinity of the carrier for glutamic acid. Reciprocal plots of velocity vs [Na] or K 1 vs 1/[Na] are linear, suggesting that glutamic acid and sodium are co‐transported on a one‐to‐one basis. t , the sodium concentration giving half maximal velocity of uptake, was found to vary from about 57 m m to 48 m m at glutamic acid concentrations of 1.0–10.0 ± 10 −6 m . A model of a mechanism by which sodium and glutamate could be co‐transported is presented; the model is in very good agreement with the experimental data.