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THE UPTAKE OF LOW CONCENTRATIONS OF LITHIUM IONS INTO RAT CEREBRAL CORTEX SLICES AND ITS DEPENDENCE ON CATIONS
Author(s) -
Wraae O.,
Hillman H.,
Round Elizabeth
Publication year - 1976
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1111/j.1471-4159.1976.tb04459.x-i1
Subject(s) - incubation , chemistry , bicarbonate , ion , lithium (medication) , saturation (graph theory) , linear relationship , analytical chemistry (journal) , nuclear chemistry , chromatography , biochemistry , endocrinology , biology , combinatorics , statistics , mathematics , organic chemistry
—Rat cerebral slices were incubated in oxygenated Krebs‐Ringer bicarbonate glucose saline, and the uptake of Li + was measured after periods of 15 s to 5 min. Saturation was not seen within the concentrations of Li + employed (0·5‐2·0 m m ). The half‐time of the uptake was 7·9 min. At steady state, after 1 h incubation, the concentration of Li + in the tissue was linearly related to that of the medium (0·5‐1·5 m m Li + ) with a concentration ratio of 1·29–1·66. The concentrations of K + and Na + in the slices incubated without Li + were found to be (μmol/g incubated wt, mean ± s.d .) 63·8 ± 9·6 and 96·2 ± 7·8 respectively (n = 28). In the presence of media with 1·5 m m ‐Li + , the K + and Na + in the slices were 56·2 ± 8·8 and 101·0 ± 7·7 respectively (n = 37). The concentration of Li + in the slices, after 1 h incubation, increased in a non linear way as the concentration of K + in the medium was decreased within a range of 0·10 m m ‐K + . In the absence of K + in the medium the uptake of Li + was approx 50% higher than in the presence of 4·9 m m ‐K + . There was an inverse linear relationship between the concentration of Li + in the slices and that of Ca 2+ in the medium within the range of 0‐5·2 m m (‐0·13 m m ‐Li + /m m Ca 2+ ). The concentration of Li + in the slices increased by approx 10% when the Mg 2+ in the medium was increased from 1·3 m m to 2·6 m m . Changes of the concentration of Na + between 120 m m and 170 m m in the medium had no significant effect on the Li + uptake.