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CONVERSION OF [1‐ 14 C]PALMITIC ACID TO [1‐ 14 C]HEXADECANOL BY DEVELOPING RAT BRAIN CELL‐FREE PREPARATIONS
Author(s) -
Natarajan V.,
Sastry P. S.
Publication year - 1976
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1111/j.1471-4159.1976.tb04443.x
Subject(s) - palmitic acid , aldehyde , stearic acid , chemistry , microsome , fatty acid , alcohol , oleic acid , cofactor , biochemistry , stereochemistry , organic chemistry , catalysis , enzyme
—The conversion of [l‐ 14 C]palmitic acid to [1‐ 14 C]hexadecanol has been demonstrated with a cell‐free system from developing rat brain. ATP, Coenzyme A and Mg 2+ were required for the activity. Fatty aldehyde was found to be an intermediate in this reaction. The conversion of fatty acid to fatty alcohol was mainly localized in the microsomal fraction and the formation of hexadecanol showed absolute specificity towards NADPH while fatty aldehyde was formed even in the absence of exogenous reduced pyridine nucleotides. The brain microsomes showed maximal activity with stearic acid and the activities with palmitic and oleic acids were 65% and 38% respectively of that with stearic acid. This enzymic reduction increased with age and showed a maximum in the 15‐day old rat brain.