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UPTAKE AND RELEASE OF NIPECOTIC ACID BY RAT BRAIN SLICES
Author(s) -
Johnston G. A. R.,
Stephanson A. L.,
Twitchin B.
Publication year - 1976
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1111/j.1471-4159.1976.tb04439.x
Subject(s) - nipecotic acid , chemistry , biophysics , potassium , sodium , biochemistry , biology , neurotransmitter , receptor , organic chemistry
—Nipecotic acid, a potent inhibitor of GABA uptake, is taken up by slices of rat cerebral cortex by a sodium‐dependent, ‘high affinity’ system ( K m 11 μ M ), and can be released from these slices by an increased potassium ion concentration in a calcium‐dependent manner. Nipecotic acid and GABA appear to be taken up by the same osmotically‐sensitive structures. GABA and substances which inhibit GABA uptake also inhibit the uptake of nipecotic acid. GABA can release preloaded nipecotic acid from brain slices, and nipecotic acid can release preloaded GABA. This indicates that GABA and nipecotic acid can be counter‐transported using the same mobile carrier. Nipecotic acid appears to have a higher affinity than GABA for this carrier.